Anti-Trimeresurus albolabris venom IgY antibodies: preparation, purification and neutralization efficacy
نویسندگان
چکیده
منابع مشابه
The effect of green pit viper (Trimeresurus albolabris) venom on platelet morphology by electron microscopy.
The incidence of venomous snake bites increases every year in Thailand, especially due to green pit viper. After the bite, there is bleeding due to thrombin-like property of the venom. The mean platelet volume has been reported to be decreased in those who have been bitten by this snake. In this study we investigate the effect of green pit viper venom (Trimeresurus albolabris) on platelet volum...
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Egg-laying hens were immunized with the Echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. The chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (PEG) and ammonium sulphate precipitation method, chloroform extraction and the Lithium sulphate precipitation method. These methods were com...
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Previously, the protective efficacy of antiAspergillus fumigatus IgY antibodies; prepared in egg laying hens, was reported in immunocompetent BALB/c mice. Since A. fumigatus infections are of main concern in immunocompromised patients, the aim of this study was to test the efficacy of the same product in Cyclosporine A treatedBALB/c mice. Heatkilled A. fumigatus was emulsified with both Freund’...
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Objective: The objective of the present investigation was to develop Chicken Egg Yolk antibodies as an alternative and affordable approach for diagnosis and treatment against dental caries in humans. Materials and Methods: Twenty four week old white leghorn chicken was immunized with formalin killed Streptococcus mutans (MTCC 890) to produce Egg Yolk antibodies (IgY). The amount of egg yolk ant...
متن کاملUnusual venom phospholipases A2 of two primitive tree vipers Trimeresurus puniceus and Trimeresurus borneensis.
To explore the venom diversity of Asian pit vipers, we investigated the structure and function of venom phospholipase A2 (PLA2) derived from two primitive tree vipers Trimeresurus puniceus and Trimeresurus borneensis. We purified six novel PLA2s from T. puniceus venom and another three from T. borneensis venom. All cDNAs encoding these PLA2s except one were cloned, and the molecular masses and ...
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ژورنال
عنوان ژورنال: Journal of Venomous Animals and Toxins including Tropical Diseases
سال: 2016
ISSN: 1678-9199
DOI: 10.1186/s40409-016-0078-3